Part:BBa_K3634011
ccdB (BsaI Removed)
The ccdAB toxin-antitoxin (TA) module is a type II TA module where the toxic ccdB protein, poisons the enzyme DNA gyrase, required for negative supercoiling of DNA (Bernard and Couturier, 1992). Through ccdB-gyrase complex formation, DNA cleavage results as well as inhibition of transcription by the formation of RNA polymerase roadblocks. The activity of the unstable ccdA antitoxin separates the ccdB-gyrase complex if present (Vandervelde et al, 2017).
The ccdB component will be used as part of the St Andrews iGEM 2020 'glucose-mediated death sensor' (BBa_K3634012), which is required to overcome intracellular ccdA concentrations causing transcriptional inhibition and DNA cleavage as well as to relieve the associated ccdAB promoter from ccdA binding to ultimately express cviJI endonuclease. These mechanisms will prevent bacterial escape into the environment and reduce the probability of horizontal gene transfer of functional protein constructs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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